CD44v4 Antibody

• Catalog Number: orb386023
• Category: Antibodies
• Description: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD44v4/1219
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the CD44v4 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD44v4 antibody is available for research use only.
• UniProt ID: P16070
• Storage: Store the CD44v4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the CD44v4 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cervical squamos cell carcinoma with CD44v4 antibody (clone CD44v4/1219). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA buffer for 10-20 min.

IHC testing of FFPE human tongue squamos cell carcinoma with CD44v4 antibody (clone CD44v4/1219). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA buffer for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD44v4 Antibody (clone CD44v4/1219). Confirmation of Integrity and Purity of the Antibody.