CD44 Antibody

• Catalog Number: orb97676
• Category: Antibodies
• Description: Mouse monoclonal to CD44.This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Hermes-3
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a ,K
• Dilution range: IHC-P: 1:10-50, WB: 1:2000, IF/ICC: 1:10-50, FACS: 1:10-50
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 81538
• Target: CD44
• UniProt ID: P16070
• NCBI: NP_001001390.1, NP_001001389.1, NP_001189486.1, NP_001001391.1, NP_001189485.1, NP_001001392.1, NP_001189484.1, NP_000601.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: anti CD44 antibody, anti LHR antibody, anti MDU2 a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-CD44 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HUVEC whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CD44 antibody confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).

CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.