CD44 Antibody

• Catalog Number: orb1333592
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD44
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI1E3
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.
• Dilution range: WB 1:400~8000, IHC 1:150, IF 1:100, FLOW 1:50
• Conjugation: Unconjugated
• MW: 79.2 kDa
• Target: CD44
• UniProt ID: P16070
• NCBI: NM_000610
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (RC221771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44. P

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody.

Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 (RC221771).

Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).