CD44 Antibody

• Catalog Number: orb1294318
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD44
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD44
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1: 100-1:200
• Conjugation: Unconjugated
• Target: CD44
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4°C.

Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4°C. Vimentin antibody (orb1294389).

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 Anti-CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 Anti-CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.