CD44 antibody

• Catalog Number: orb1151854
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD44
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rHCAM/6449
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD44 antibody is available for research use only.
• UniProt ID: P16070
• Storage: Aliquot the CD44 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD44 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human esophagus tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using CD44 antibody (clone rHCAM/6449) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.