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Catalog Number | orb1151545 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD44 |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | HCAM/6459R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | Recombinant full-length human CD44 protein was used as the immunogen for the recombinant CD44 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant CD44 antibody is available for research use only. |
UniProt ID | P16070 |
Storage | Aliquot the recombinant CD44 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant CD44 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE analysis of purified, BSA-free recombinant CD44 antibody (clone HCAM/6459R) as confirmation of integrity and purity.
IHC staining of FFPE human breast tissue with recombinant CD44 antibody (clone HCAM/6459R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophagus tissue with recombinant CD44 antibody (clone HCAM/6459R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD44 antibody (clone HCAM/6459R) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
ELISA, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
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