CD43 Antibody

• Catalog Number: orb248547
• Category: Antibodies
• Description: This antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with CD20 antibody, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with CD20 and CD42 antibody argues against a reactive process and favors a diagnosis of lymphoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DF-T1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Myeloblastic KG1 cells were used as the immunogen.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD43 antibody is available for research use only.
• Entrez: 6693
• Storage: Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen with CD43 antibody (clone DF-T1).

FACS staining of human lymphocytes using CD43 antibody (red) and isotype control.

Western blot testing of human K562 cell lysate with CD43 antibody (clone DF-T1). Predicted molecular weight 45-135 kDa depending on glycosylation level.

Immunofluorescence staining of human K562 cells with CD43 antibody (clone DF-T1, green) and NucSpot (red).

SDS-PAGE analysis of purified, BSA-free CD43 antibody (clone DF-T1) as confirmation of integrity and purity.

Flow cytometry staining of PFA-fixed human K562 cells with CD43 antibody; Red=isotype control, Blue=CD43 antibody.