CD40LG Antibody

• Catalog Number: orb1671566
• Category: Antibodies
• Description: CD40LG Antibody
• Clonality: Recombinant
• Clone Number: chi220
• Tested applications: Blocking
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD40LG
• UniProt ID: P29965
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFSF5, CDw40, TRAP, p50, Bp50, CD40L receptor, Tu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-CD40 antibody chi220. Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of chi220 (orb1671566 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody chi220. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of chi220 (orb1671566) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671566- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.