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Catalog Number | orb758981 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD40 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | chi220 |
Tested applications | Blocking |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD40 |
UniProt ID | P29965 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | TNFSF5; CDw40; TRAP; p50; Bp50; CD40L receptor; Tu Read more... |
Note | For research use only |
Application notes | This antibody blocks the interaction between CD40 and gp39. Chi220 was used either alone or in combination with CTLA4-Ig in an immunosuppresive therapy study to check the survival of renal allografts in non human primate models (PMID: 12394833). Chi220 blocks binding of CD154, it also has partial agonist properties and weak stimulatory potential. It synergisticaly works with LEA29Y (belatacept) to prolong the islet allograft survival in a non-human primate Macaca fascicularis (PMID: 15611281). A study on protection of islets xenographs in diabetic macaques used a combination of Chi220 with IL-2 receptor specific antibody Basiliximab for immunosuppression with induction therapy. Chi220 is used as an sCD154 blocking agent and it was seen that it binds sCD154 in a dose dependant manner (PMID: 21920020). Two humanized versions of this antibody were generated F4 and L3.17, also showed similar properties. Humanized versions of this antibody are available on request. |
Expiration Date | 12 months from date of receipt. |
ELISA, FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Porcine | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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