CD40 Antibody

• Catalog Number: orb606660
• Category: Antibodies
• Description: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C40/2383
• Tested applications: ELISA, FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 29-107 from the human protein was used as the immunogen for the CD40 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD40 antibody is available for research use only.
• UniProt ID: P25942
• Storage: Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD40 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil with CD40 antibody (clone C40/2383). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Flow cytometry testing of human U-2 OS cells with CD40 antibody (clone C40/2383); Red=isotype control, Blue= CD40 antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD40 antibody (clone C40/2383). These results demonstrate the foremost specificity of the C40/2383 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD40 antibody (clone C40/2383) as confirmation of integrity and purity.