CD4 Antibody

• Catalog Number: orb699617
• Category: Antibodies
• Description: Recognizes a protein of 55kDa, identified as CD4. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rC4/206
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CD4 protein was used as the immunogen for the recombinant CD4 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD4 antibody is available for research use only.
• UniProt ID: P01730
• Storage: Store the recombinant CD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD4 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human PBMC with recombinant CD4 antibody (clone rC4/206, green).

Flow cytometry testing of human PBMC with recombinant CD4 antibody (clone C40/2383); Red=isotype control, Green= recombinant CD4 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD4 antibody (clone rC4/206) as confirmation of integrity and purity.