CD4 Antibody

• Catalog Number: orb1259488
• Category: Antibodies
• Description: CD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD4 (NP_000607.1).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: CD4
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CD4 Antibody [9H5A8] : CD4mut
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CD4 antibody (orb1259488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.