CD38 antibody

• Catalog Number: orb1151666
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD38
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD38/4328
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CD38 protein was used as the immunogen for the CD38 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/million cells,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD38 antibody is available for research use only.
• UniProt ID: P28907
• Storage: Aliquot the CD38 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD38 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) spleen and 2) Raji cell lysate using CD38 antibody (clone CD384328). Expected molecular weight: 34-46 kDa depending on glycosylation level.

Western blot testing of human Raji cell lysate using CD38 antibody (clone CD38/4328). Expected molecular weight: 34-46 kDa depending on glycosylation level.

IHC staining of FFPE human tonsil tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human cerebral cortex tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD38 antibody (clone CD38/4328). These results demonstrate the foremost specificity of the CD38/4328 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.