CD38 antibody

• Catalog Number: orb1151661
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD38
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CD38/6448R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 200-300 of human CD38 protein was used as the immunogen for the recombinant CD38 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD38 antibody is available for research use only.
• UniProt ID: P28907
• Storage: Aliquot the recombinant CD38 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD38 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate adenocarcinoma tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji cell lysate using recombinant CD38 antibody (clone CD38/6448R). Expected molecular weight: 34-46 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free recombinant CD38 antibody (clone CD38/6448R) as confirmation of integrity and purity.