CD37 Antibody

• Catalog Number: orb1671483
• Category: Antibodies
• Description: CD37 Antibody
• Clonality: Recombinant
• Clone Number: G28-1
• Tested applications: Depletion
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing mice with CD37.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD37
• UniProt ID: P11049
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TSPAN-26, Leukocyte antigen CD37, Tetraspanin-26,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD37 antibody G28-1 (Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of G28-1 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody G28-1 (Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of G28-1 (orb1671483) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml) - showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671483 - DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.