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    CD35 antibody

    Catalog Number: orb248915

    DispatchUsually dispatched within 5-10 working days
    $ 267.00
    Catalog Numberorb248915
    CategoryAntibodies
    DescriptionMouse monoclonal antibody to CD35
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberE11
    Tested applicationsFC, IF
    ReactivityHuman
    ImmunogenNative purified human CD35.
    Form/AppearanceMouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
    ConjugationUnconjugated
    TargetCR1
    Entrez1378
    UniProt IDP17927
    SourceMouse
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesMouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
    Alternative namesanti C3 binding protein antibody, anti C3b/C4b rec
    Read more...
    NoteFor research use only
    Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    Expiration Date12 months from date of receipt.
    CD35 antibody

    Flow cytometric analysis of human peripheral blood granulocytes using Anti-CD35 Antibody, followed by anti-mouse IgG PE.

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