CD35 antibody (FITC)

• Catalog Number: orb154494
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD35 conjugated to FITC.
• Clonality: Monoclonal
• Clone Number: E11
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Acute monocytic leukemia cells and normal blood monocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD35
• Entrez: 1378
• UniProt ID: P17927
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti C3 binding protein antibody (FITC), anti C3b/
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from CD35 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD35 (E11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD35 (E11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).