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Catalog Number | orb1993652 |
---|---|
Category | Antibodies |
Description | CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. |
Clonality | Monoclonal |
Clone Number | 4H11[APG] |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia. |
Conjugation | PerCP |
Target | CD34 |
Entrez | 947 |
UniProt ID | P28906 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).
FC, IF | |
Human | |
Mouse | |
Monoclonal | |
PerCP/Cy5.5 |
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