CD34 antibody

• Catalog Number: orb44481
• Category: Antibodies
• Description: Mouse Monoclonal to CD34.
• Clonality: Monoclonal
• Clone Number: QBEnd-10
• Tested applications: FA, FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Human endothelial vesicles
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line. Flow cytometry: Recommended dilution: 5 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD34
• Entrez: 947
• UniProt ID: P28906
• RRID: AB_11013298
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-CD34 antibody, anti-Mucosialin antibody
• Note: For research use only
• Application notes: Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line. Flow cytometry: Recommended dilution: 5 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of CD34 in human peripheral blood with anti-CD34 (QBEnd-10) azide free.

Flow cytometry multicolor surface staining of human peripheral blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0.6 µg/ml, GAM APC, red-filled) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD45dim CD34 positive stem cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Western blotting analysis of human CD34 using mouse monoclonal antibody QBEnd-10 on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.