CD315 Antibody

• Catalog Number: orb1678983
• Category: Antibodies
• Description: CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
• Clonality: Monoclonal
• Clone Number: 1F11
• Tested applications: FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: HeLa cells
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD315
• Entrez: 5738
• UniProt ID: Q9P2B2
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells stained using anti-human CD315 (1F11) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from A431 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).