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Catalog Number | orb154374 |
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Category | Antibodies |
Description | Mouse monoclonal antibody to CD314 which is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion. |
Clonality | Monoclonal |
Clone Number | 1D11 |
Tested applications | FA, FC, IHC-Fr, IP |
Reactivity | Human |
Isotype | Mouse IgG1 kappa |
Immunogen | NKL cell line |
Concentration | 1 mg/ml |
Dilution range | Functional application: Blocking of ligand binding. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD314 |
Entrez | 22914 |
UniProt ID | P26718 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | Anti-NKG2D antibody, anti-KLRK1 antibody, anti-KLR Read more... |
Note | For research use only |
Application notes | Functional application: Blocking of ligand binding. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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