CD31 Mouse Monoclonal Antibody

• Catalog Number: orb500839
• Category: Antibodies
• Description: CD31 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2D4
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human CD31 28-601/727aa (C-6x His-Tag)
• Concentration: 1mg/ml
• Dilution range: WB=1:100-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.5ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: PECAM1
• UniProt ID: P16284
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: platelet endothelial cell adhesion molecule precur
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fábio Vieira de Miranda-Filho 1, Stéfany Barbosa 1, Olavo Alcalde Panigali 1, Mirela Caroline Silva 1, Monique Gonçalves da Costa 1, Franciele da Silva Flores 1, Edilson Ervolino 2, Letícia Helena Theodoro 1, Osvaldo Magro-Filho 1, Leonardo Perez Faverani Effect of local and systemic administration of atorvastatin for improving bone healing on critical defects Braz Dent J ., (2024)

PubMed: 39476113

Applications

IHC

Reactivity

Rat

25 ug total protein per lane of various lysates probed with CD31 monoclonal antibody, unconjugated (orb500839) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (orb500839) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (orb500839) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, DAPI (blue) was used to stain the cell nuclei.