CD31 Antibody / PECAM-1

• Catalog Number: orb750177
• Category: Antibodies
• Description: CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C31/1395R
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CD31 antibody is available for research use only.
• Entrez: 5175
• UniProt ID: P16284
• Storage: Store the recombinant CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant CD31 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone C31/1395R). Expected molecular weight: 83-130 kDa depending on level of glycosylation.

Flow cytometry testing of PFA-fixed human Jurkat cells with recombinant CD31 antibody (clone C31/1395R); Red=isotype control, Blue= recombinant CD31 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD31 antibody (clone C31/1395R) as confirmation of integrity and purity.