CD31 Antibody / PECAM-1

• Catalog Number: orb750004
• Category: Antibodies
• Description: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: JC/70A
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD31 antibody is available for research use only.
• UniProt ID: P16284
• Storage: Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the CD31 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with CD31 antibody (clone JC/70A).

IHC analysis of formalin-fixed, paraffin-embedded human angiosarcoma stained with CD31 antibody (clone JC/70A).

Flow cytometry testing of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled CD31 antibody (JC/70A).

Western blot testing of ThP1 cell lysate with CD31 antibody (clone JC/70A). Predicted size: 83-130KD depending on level of glycosylation.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD31 antibody (blue, clone JC/70A) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free CD31 antibody (clone JC/70A) as confirmation of integrity and purity.