CD31 antibody

• Catalog Number: orb1151843
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD31
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PECAM1/3534
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 625-738 was used as the immunogen for the PECAM1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PECAM1 antibody is available for research use only.
• UniProt ID: P16284
• Storage: Aliquot the PECAM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PECAM1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of human Jurkat cells with PECAM1 antibody (blue, clone PECAM1/3534) and isotype control (red).

IHC staining of FFPE human tonsil tissue with PECAM1 antibody (clone PECAM1/3534). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with PECAM1 antibody (clone PECAM1/3534). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human THP-1 cell lysate using PECAM1 antibody (clone PECAM1/3534). Expected molecular weight: 83-130 kDa depending on level of glycosylation.

Immunofluorescent staining of human Jurkat cells using PECAM1 antibody (green, clone PECAM1/3534) and RedDot (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD31 antibody (clone PECAM1/3534). These results demonstrate the foremost specificity of the PECAM1/3534 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.