CD31 antibody

• Catalog Number: orb10314
• Category: Antibodies
• Description: CD31 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD31. Please contact us for the exact immunogen sequence. The peptide is available as orb374712.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB:2 μg/ml, IHC-P:1:500, IF/ICC: 1:200
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 82 kDa
• Target: CD31
• Entrez: 5175
• UniProt ID: Q08481, Q3SWT0, P16284
• NCBI: 41, 000433, 000442
• RRID: AB_10751280
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Adhesion molecule antibody, anti CD31 antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hideo Kagaya 1, Alexander S Kim 1, Mian Chen 1, Pei-Yu Lin 1, Xuanzhi Yin 2, Matthew Spite 2, Michael S Conte Dynamic changes in proresolving lipid mediators and their receptors following acute vascular injury in male rats Physiol Rep, (2024)

PubMed: 39128880

Applications

ICC

Reactivity

Rat

Geórgia da Silva Feltran 1, Rodrigo Augusto da Silva 2, Célio Junior da Costa Fernandes 1, Marcel Rodrigues Ferreira 1, Sérgio Alexandre Alcântara Dos Santos 3, Luis Antônio Justulin Junior 3, Liliana Del Valle Sosa 4, Willian Fernando Zambuzzi Vascular smooth muscle cells exhibit elevated hypoxia-inducible Factor-1α expression in human blood vessel organoids, influencing osteogenic performance Exp Cell Res, (2024)

PubMed: 38909881

Applications

IF

Reactivity

Human

Jelodari, Sahar et al. Assessment of the Efficacy of an LL-37-Encapsulated Keratin Hydrogel for the Treatment of Full-Thickness Wounds ACS Appl Bio Mater, (2023)

PubMed: 37224450

Mai, Jingting et al. Tachycardia pacing induces myocardial neovascularization and mobilizes circulating endothelial progenitor cells partly via SDF-1 pathway in canines Heart Vessels, 31, 230-240 (2016)

PubMed: 25491934

Fernandez-Moure, Joseph S. et al. Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair Wound Repair Regen, 25, 98-108 (2017)

PubMed: 28076885

Valipour, Elahe et al. Anti-angiogenic effects of testis-specific gene antigen 10 on primary endothelial cells Gene, 754, 144856 (2020)

PubMed: 32512160

Raisi, Abbas et al. Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats BMC Complement Med Ther, 21, 126 (2021)

PubMed: 33879143

IHC-P image of mouse kidney tissue using CD31 antibody (dilution of primary antibody at 1:500)

Immunohistochemical staining of paraffin embedded mouse skin tissue using anti-CD31 (primary antibody at 1:200)

IHC-P staining of mouse lung tissue using CD31 antibody (dilution at 1:200)

Immunohistochemical staining of paraffin embedded rat brain tissue using CD31 antibody (primary antibody at 1:100)

Immunohistochemical staining of mouse lung tissue using CD31 antibody (dilution of primary antibody - 1:200)

Western blot analysis of rat heart (lane 1), uterus (lane 2), ovary (lane 3), lung (lane4), trachea (lane 5) using CD31 antibody (primary antibody at 2 ug/ml)

Immunofluorescense analysis of mouse lung tissue using anti-CD31 (primary antibody at 1:200)

IHC-P staining of mouse lung tissue using CD31 antibody (dilution at 1:200)

IF analysis of mouse lung tissue using anti-CD31 (dilution of primary antibody at 1:200)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD31 antibody (primary antibody at 1:200)

Immunohistochemical staining of rat heart tissue using anti-CD31 (primary antibody at 1:100)

WB analysis of rat heart (lane 1), uterus (lane 2), ovary (lane 3), lung (lane4), trachea (lane 5) using CD31 antibody (dilution of primary antibody - 2 ug/ml)

IF analysis of rat kidney tissue using anti-CD31 (dilution of primary antibody at 1:100)

IHC-P image of rat skin tissue using CD31 antibody (dilution of primary antibody at 1:100)

IF analysis of rat lung tissue using anti-CD31 (dilution of primary antibody at 1:100)

IHC-P staining of rat lung tissue using anti-CD31 (dilution at 1:100)

IHC-P staining of mouse skin tissue using anti-CD31 (dilution at 1:100)

IHC-P staining of mouse kidney tissue using anti-CD31 (dilution at 1:100)

Immunofluorescence analysis of rat skin tissue using CD31 antibody (dilution of primary antibody - 1:100)

IHC-P staining of mouse skin tissue using CD31 antibody (dilution at 1:100)