CD3 epsilon Antibody

• Catalog Number: orb639538
• Category: Antibodies
• Description: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C3e/3125R
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A recombinant human partial protein (amino acids 23-119) was used as the immunogen for the recombinant CD3e antibody.
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD3e antibody is available for research use only.
• UniProt ID: P07766
• Storage: Store the recombinant CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MOLT-4 cell lysate with recombinant CD3e antibody (clone C3e/3125R). Predicted molecular weight ~23 kDa.

IHC staining of FFPE human tonsil with recombinant CD3e antibody (clone C3e/3125R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD3e antibody (clone C3e/3125R) as confirmation of integrity and purity.