CD3 epsilon Antibody

• Catalog Number: orb606527
• Category: Antibodies
• Description: This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C3e/2478
• Tested applications: ELISA, FACS, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 23-119 were used as the immunogen for the CD3e antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD3e antibody is available for research use only.
• UniProt ID: P07766
• Storage: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD3e antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/2478); Red=isotype control, Blue= CD3e antibody.

Western blot testing of human Jurkat cells with CD3e antibody (clone C3e/2478). Expected molecular weight ~23 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD3e antibody (clone C3e/2478). These results demonstrate the foremost specificity of the C3e/2478 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone C3e/2478) as confirmation of integrity and purity.