CD28 Antibody [204.12]

• Catalog Number: orb1252438
• Category: Antibodies
• Description: CD28 Antibody [204.12]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 204.12
• Tested applications: FA, FC, IF
• Reactivity: Bovine, Human, Mouse, Porcine, Sheep
• Isotype: IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD28
• UniProt ID: P10747
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD4 Antibody [9H5A8] : CD4mut
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlFunctional Activity (Order SAF formulation)Optimal dilution of the CD28 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).

Flow cytometry testing of paraformaldehyde-fixed human Jurkat cells with CD28 antibody (clone 204.12); Red = isotype control, Blue = CD28 antibody.

SDS-PAGE analysis of purified, BSA-free CD28 antibody (clone 204.12) as confirmation of integrity and purity.