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Catalog Number | orb389314 |
---|---|
Category | Antibodies |
Description | CD25 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human CD25. Please contact us for the exact immunogen sequence. The peptide is available as orb13468. |
Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
Dilution range | WB: 1:100-500, IHC-P: 1:100-500, IF/ICC: 1:100-500 |
Form/Appearance | 10 mM PBS, 0.02% sodium azide |
Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Conjugation | Unconjugated |
MW | 31 kDa |
Target | CD25 |
UniProt ID | P01589, P01590, P25094 |
NCBI | 000408 |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of paraffin embedded rat spleen tissue using CD25 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat lymph node tissue using CD25 antibody (2.5 ug/ml)
IF image of rat lymph node tissue using anti-CD25 (2.5 ug/ml)
Western blot analysis of rat lipocyte (lane 1), rat spleen (lane 2), rat small intestines (lane 3), rat lymph node (lane 4), hela cells (lane 5), human lung cancer (lane 6) using CD25 antibody (1 ug/ml)
IHC-P image of human ovarian cancer tissue using anti-CD25 (2.5 ug/ml)
IF analysis of rat lymph node tissue using anti-CD25 (2.5 ug/ml)
Immunohistochemical staining of human lung cancer tissue using CD25 antibody (2.5 ug/ml)
ELISA, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
Blocking, FC, IF, IHC, WB | |
Human, Primate | |
Recombinant | |
Unconjugated |
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