CD23 antibody

• Catalog Number: orb2276273
• Category: Antibodies
• Description: CD23 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-023M
• Tested applications: IHC
• Reactivity: Human
• Isotype: gG1, kappa
• Immunogen: Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P06734
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BLAST-2; C-type lectin domain family 4, member J;
• Note: For research use only
• Application notes: Positive Control: Tonsil: The follicular dendritic cells of the germinal centres should show a strong CD23 immunostaining while the majority of lymphocytes in the mantle zone of the follicles should show an at least weak staining. Negative Control: Tonsil: The squamous epithelial cells and T-cells in the interfollicular T-zones should not show any CD23 immunostaining. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

B cell chronic lymphatic leukemia B CLL composed of strongly CD23 positive tumor cells.

CD23 negative mantle cell lymphoma containing few germinal centre remnants that include CD23 positive dendritic cells.

Lymph node In lymph nodes a strong CD23 immunostaining is seen in follicular dendritic cells of germinal centres while staining is moderate in follicular and perifollicular B lymphocytes.