CD229 antibody

• Catalog Number: orb256660
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD229
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD229. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LY9
• Entrez: 17085, 4063
• UniProt ID: Q01965, Q9HBG7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti T-lymphocyte surface antigen Ly-9 antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD229 expression in SGC7901 (A), HCT116 (B), HUT78 (C) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 110 kD)

Immunohistochemical analysis of CD229 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.