CD207 Antibody

• Catalog Number: orb1806417
• Category: Antibodies
• Description: CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.
• Clonality: Monoclonal
• Clone Number: 2G3
• Tested applications: ELISA, FC, ICC, IHC-Fr
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 lambda
• Immunogen: Fusion protein of human CD207 extracellular part and IgG Fc fragment
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD207
• Entrez: 50489
• UniProt ID: Q9UJ71
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: CLEC4K, langerin
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor intracellular staining pattern of human stimulated (GM-CSF + IL-4 + TGF-beta) monocytes using anti-human CD207 (2G3) purified antibody (concentration in sample 0.56 μg/ml, GAM APC) and anti-human CD64 (10.1) Pacific Blue™ antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD64 positive CD207 positive cells (red-filled) from CD64 negative CD207 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (GM-CSF + IL-4 + TGF-beta) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human stimulated (GM-CSF + IL-4 + TGF-beta) peripheral blood mononuclear cells whole blood stained using anti-human CD207 (2G3) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).