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Catalog Number | orb386222 |
---|---|
Category | Antibodies |
Description | CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | IGEL/1497R |
Tested applications | FACS, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant CD20 antibody is available for research use only. |
UniProt ID | P11836 |
Storage | Recombinant CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.
Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone IGEL/1497R); Red=isotype control, Blue= recombinant CD20 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant CD20 antibody (clone IGEL/1497R) as confirmation of integrity and purity.
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