CD20 antibody

• Catalog Number: orb2276515
• Category: Antibodies
• Description: CD20 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-020R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human full-length MS4A1 protein
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P11836
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: APY; ATOPY; B-lymphocyte cell-surface antigen B1;
• Note: For research use only
• Application notes: Positive Control: Tonsil or appendix: A strong, predominantly membranous staining of the mantle zone B-cells, the germinal centre B- cells and the interfollicular B-cells is required, while all other cells should stain negative. Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any staining. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Strong CD20 staining of a germinal centre in the appendix mucosa while epithelial cells are completely negative.

The thymus contains a relatively low fraction of CD20 positive B-lymphocytes.

In the tonsil, many lymphocytes stain positive while the surface epithelium is CD20 negative.