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Catalog Number | orb1826068 |
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Category | Antibodies |
Description | Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This mAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | MS4A1/8072R |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant partial protein (within amino acids 200-297) from the human protein was used as the immunogen for the recombinant CD20 antibody. |
Dilution range | Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant CD20 antibody is available for research use only. |
UniProt ID | P11836 |
Storage | Aliquot the recombinant CD20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human tonsil tissue with recombinant CD20 antibody (clone MS4A1/8072R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with recombinant CD20 antibody (clone MS4A1/8072R). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant CD20 antibody (clone MS4A1/8072R) as confirmation of integrity and purity.
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