You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1672191 |
---|---|
Category | Antibodies |
Description | CD2 Antibody |
Clonality | Recombinant |
Clone Number | YTH 655 |
Tested applications | Depletion, FC |
Reactivity | Human |
Isotype | IgG kappa |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | CD2 |
UniProt ID | P06729 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | LFA-2, T-cell surface antigen CD2, Erythrocyte rec Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot using anti-CD2 antibody YTH 655. Human tonsil lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of YTH 655 (orb1672191) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa- but this protein is glycosylated at several residues. orb1672191 successfully detected CD2 in human tonsil lysate.
Immunofluorescence staining of fixed Molt4 cells with anti-CD2 antibody YTH 655. Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of YTH 655 (orb1672191) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane- and some cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672191- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating