CD1a Antibody

• Catalog Number: orb750173
• Category: Antibodies
• Description: CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein expressed in association with Beta-2 microglobulin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C1A/1506R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length human CD1a protein was used as the immunogen for this recombinant CD1a antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CD1a antibody is available for research use only.
• Entrez: 909
• Storage: Store the recombinant CD1a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD1a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human skin with recombinant CD1a antibody (clone C1A/1506R). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CD1a antibody (clone C1A/1506R) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant CD1a antibody (clone C1A/1506R). These results demonstrate the foremost specificity of the C1A/1506R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.