CD19 Antibody / B-lymphocyte marker

• Catalog Number: orb749433
• Category: Antibodies
• Description: CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CVID3/429
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD19 antibody is available for research use only.
• UniProt ID: P15391
• Storage: CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with CD19 antibody (clone CVID3/429); Red=isotype control, Blue=CD19 antibody.

Flow cytometry testing of lymphocyte-gated human PBM cells with CD19 antibody (clone CVID3/429); Gray=unstained cells, Violet=anti-CD19 antibody stained cells.

SDS-PAGE analysis of purified, BSA-free CD19 antibody (clone CVID3/429) as confirmation of integrity and purity.