CD19 Antibody / B-lymphocyte marker

• Catalog Number: orb749432
• Category: Antibodies
• Description: CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. It is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CVID3/155
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human CD19 recombinant protein was used as immunogen for this anti-CD19 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-CD19 antibody is available for research use only.
• UniProt ID: P15391
• Storage: Anti-CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD19 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of lymphocyte-gated human PBM cells with anti-CD19 antibody (clone CVID3/155); Gray=unstained cells, Orange=anti-CD19 antibody stained cells.

Flow cytometry testing of human Raji cells with anti-CD19 antibody (clone CVID3/155); Red=isotype control, Blue=anti-CD19 antibody.

Immunofluorescent staining of human Raji cells with anti-CD19 antibody (clone CVID3/155, green) and Reddot nuclear stain (red).

SDS-PAGE Analysis of Purified, BSA-Free CD19 Antibody (clone CVID3/155). Confirmation of Integrity and Purity of the Antibody.