CD18 antibody

• Catalog Number: orb154416
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1). CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression.
• Clonality: Monoclonal
• Clone Number: MEM-48
• Tested applications: FA, FC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex. Western blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD18
• Entrez: 3689
• UniProt ID: P05107
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-Integrin beta2 antibody, anti-Complement rece
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).