You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
CD171/L1CAM Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1600350
| Catalog Number | orb1600350 |
|---|---|
| Category | Antibodies |
| Description | CD171/L1CAM Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 1C3 |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Human |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | Recombinant human L1CAM protein |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 138 kDa |
| Target | L1CAM |
| UniProt ID | P32004 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 1*TBS (pH7.4), 0.05% rAlbumin, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Alternative names | L1CAM_HUMAN; Neural cell adhesion molecule L1; CAM Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of L1CAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: Human brain tissue lysate.
