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CD163L1 Antibody (C-term)
Catalog Number: orb1936245
| Catalog Number | orb1936245 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB33379 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 159239 Da |
| Target | This CD163L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1401-1430 amino acids from the C-terminal region of human CD163L1. |
| UniProt ID | Q9NR16 |
| NCBI | NP_777601.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Scavenger receptor cysteine-rich type 1 protein M1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-CD163L1 Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 159 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis on paraffin-embedded Human spleen tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis on paraffin-embedded Human lymph node tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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