CD163 Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb124511
• Category: Antibodies
• Description: CD163 Rabbit Polyclonal Antibody (PE)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF
• Predicted Reactivity: Canine, Equine, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD163 (1001-1121/1156aa)
• Concentration: 1mg/ml
• Dilution range: IF=1:100-500
• Conjugation: PE
• Target: CD163
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Scavenger receptor cysteine-rich type 1 protein M1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: JEG-3. Primary Antibody (green line): Rabbit Anti-CD163 antibody (orb124511), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD163 antibody (orb124511), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.