CD163 antibody

• Catalog Number: orb44713
• Category: Antibodies
• Description: Mouse monoclonal to CD163. CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.
• Clonality: Monoclonal
• Clone Number: GHI/61
• Tested applications: FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Hairy cell leukemia cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD163
• Entrez: 9332
• UniProt ID: Q86VB7
• RRID: AB_10990688
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-Cluster Of Differentiation antibody, anti-CD
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD163 (GHI/61) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD163 (GHI/61) purified antibody (concentration in sample 2 µg/ml) GAM APC.