CD16 antibody (FITC)

• Catalog Number: orb44104
• Category: Antibodies
• Description: Mouse Monoclonal to CD16.
• Clonality: Monoclonal
• Clone Number: MEM-154
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human granulocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD16
• RRID: AB_10991462
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD16 antibody, anti-FACSgammaRIII antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Reactivity of Anti-human CD16 FITC antibody (clone MEM-154) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD16 FITC antibody (clone MEM-154, 6.7 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition. Donor of tested blood sample had 158F polymorphism on CD16a.

Anti-human CD16 FITC antibody (clone MEM-154) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 FITC antibody (clone MEM-154) was used in concentration 6.7 µg/ml in stained blood sample (2 x 10^6 cells). Donor of tested blood sample had 158F polymorphism on CD16a.