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Catalog Number | orb1473966 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD138 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, IF, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide of human CD138. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000) |
Form/Appearance | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | SDC1 |
Entrez | 6382 |
UniProt ID | P18827 |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | SDC; Syndecan-1; SYND1; CD138 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD138 expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 70 kD)
Immunofluorescent analysis of CD138 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HepG2 cells using Anti-CD138 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
IF, IHC, WB | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Bovine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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