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Catalog Number | orb750323 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD137L conjugated to Biotin |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CD137L/1547 |
Tested applications | FACS, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2a, kappa |
Immunogen | Recombinant full-length human CD137L protein was used as the immunogen for the CD137L antibody. |
Dilution range | Flow cytometry: 2-4ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT |
Purity | Protein G affinity chromatography |
Conjugation | Biotin |
Formula | 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This CD137L antibody is available for research use only. |
UniProt ID | P41273 |
Storage | Store the CD137L antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term). |
Buffer/Preservatives | 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the CD137L antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human kidney with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD137L antibody as confirmation of integrity and purity.
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
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