CD137 antibody (Biotin)

• Catalog Number: orb750317
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD137 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4-1BB/3201
• Tested applications: ELISA, FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 19-188 from the human protein was used as the immunogen for the CD137 antibody.
• Dilution range: ELISA (order Ab without BSA for coating),Immunofluorescence: 2-4ug/ml,Flow cytometry: 2-4ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Biotin
• Formula: 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD137 antibody is available for research use only.
• UniProt ID: Q07011
• Storage: Store the CD137 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD137 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human renal cell carcinoma with biotinylated CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with biotinylated CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD137 antibody (clone 4-1BB/3201). These results demonstrate the foremost specificity of the 4-1BB/3201 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.