CD137 antibody (APC)

• Catalog Number: orb421964
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD137 conjugated to APC
• Clonality: Monoclonal
• Clone Number: 4B4-1
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant human CD137 ectodomain
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD137
• Entrez: 3604
• UniProt ID: Q07011
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TNFRSF9 antibody, anti-41BB antibody, anti-4-
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human peripheral blood mononulcear cells stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD25 (MEM-181) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of cells stained using anti-human CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1 μg/ml, same as CD137 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension.

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).